An endpoint method counts 8 of 10 Sholl intersections at 10 µm in mouse trace 314642645.

We compared an endpoint rule with an analytic line–sphere intersection calculation on dendrite centerlines from 12 public mouse neuron traces selected by specimen ID. At 10 µm in specimen 314642645, the endpoint rule counted 8 intersections and the analytic rule counted 10 because one segment crossed the sphere twice with both endpoints outside. Across the primary 10 µm radius grid, totals were 2,685 and 2,687, with one differing cell–radius pair among 448. A secondary 1 µm grid found the same event and no additional event. All 11 synthetic checks passed; Decimal arithmetic at 80 digits confirmed the differing geometry. The sample is small and nonrandom, and the result concerns stored straight centerlines. It does not establish tracing accuracy, population error rates, cell function, or biological effects. The Allen SWC files and raw API responses remain available from the source URLs and are identified by file IDs and SHA-256 hashes in the publication archive; they are not redistributed under SCI terms.

Sholl analysis · neuron morphology · dendrite · segment intersection · sphere crossing · Allen Cell Types Database · numerical validation