CxHP8 activation gave backward left front tarsus shifts in nine male flies with forward motion before light onset

We tested a movement direction predicted from a fly connectome. The published CxHP8 hair-plate circuit predicts backward leg motion. We used fixed activation and genetic-light control data from Pratt et al. We selected rest trials by behavior before light onset and retained all later behavior. With the source 0.4 mm coxa scale, at 100–200 ms after onset, the equal-animal activation-minus-control tarsus shift was -0.450 mm, with a 95% whole-animal bootstrap interval of -0.624 to -0.283 mm. The rest set had nine activation flies and eight control flies. A specified set with mean forward speed above 5 mm/s before onset had nine flies in each group. Its shift was -0.754 mm, with interval -1.000 to -0.497 mm. All nine activation flies had backward on-minus-off shifts in both sets. Shared trial codes, minimum trial counts, coordinate changes and single-animal removals kept the backward direction. Starting position had a negative interaction in both genetic groups; their difference remained uncertain. These data extend the observed direction to trials that begin with forward motion. They do not identify the active synaptic route or establish a state-specific neural gain change. Groups were recorded on different dates. Trial assignment and extra condition codes remain unresolved. The package preserves new code, derived data, full-precision curves, animal results, figures, source hashes and repeat instructions. Original observations remain credited to Pratt and colleagues.

connectome · proprioception · CxHP8 · Drosophila · sensorimotor reflex · behavioral state · trial selection · optogenetics · reproducibility