The mean backward CxHP8 response remained in late repetition labels in nine male flies

We tested whether a movement response predicted from a fly connectome changed across repeated stimulation. We used fixed CxHP8 activation data from nine male flies and genetic-light controls from nine male flies. The main panel had 1,421 complete trials. Within each fly and repetition set, four on codes and four off codes had equal weight. Flies also had equal weight. The outcome was backward left front tarsus displacement at 100–200 ms after onset. Repetition labels 1–5 and 6–10 defined the two sets. One activation fly had late labels 6–8 only. With the source 0.4 mm coxa scale, the late activation-minus-control mean was -0.477 mm, with a 95% fly bootstrap interval of -0.651 to -0.308 mm. The early mean was -0.513 mm. The late-minus-early change was +0.037 mm, with interval -0.156 to +0.234 mm. A Welch interval was -0.190 to +0.263 mm. Thus, the predicted movement direction remained in the group mean, but the response change was uncertain. Position and motion adjustment gave a change of -0.005 mm, with interval -0.119 to +0.109 mm. The data do not establish constant response size. The standing subset was too sparse for the planned balanced test. Genotype and recording date differ, code meanings are incomplete, and acquisition timing is not validated. The study does not identify habituation or a synaptic cause. The package has code, trial data, curves, source hashes, all fly estimates, figures, and repeat instructions. Raw extraction, separate coordinate calculations, and a clean offline replay passed.

connectome · proprioception · CxHP8 · Drosophila · repeated stimulation · sensorimotor reflex · animal-level inference