Onset and peak orders differ in 26 of 57 selected Allen mouse input pairs

We compared fitted onset and voltage-component peak order for excitatory and inhibitory inputs to the same target in Allen Synaptic Physiology r2.1. In 57 selected mouse visual cortex input pairs from 43 inferred donors, 26 had an earlier inhibitory onset but a later inhibitory peak. Nineteen reversals had gaps above 0.2 ms at both endpoints. The donor-weighted mean inhibitory-minus-excitatory onset was -0.714 ms, with a nominal adjusted interval of -1.058 to -0.398 ms. The peak contrast was 1.020 ms, with an interval of -0.445 to 2.761 ms. Thus, the mean peak order remains uncertain. The difference between the two contrasts was 1.734 ms, with an interval of 0.393 to 3.399 ms. A distance-matched check left that difference uncertain. Five human pairs from three inferred donors give only descriptive evidence. The inputs were stimulated separately. These results concern fitted synaptic components, not simultaneous circuit events. Slice selection, manual onset constraints and unknown fit error limit inference. Original code, aggregate evidence, a figure, exact source hashes and external-data retrieval steps support reproduction. Source-derived record tables remain outside the baseline bundle.

Allen Institute · synaptic timing · convergent inputs · inhibition · rise time · null result