The HAP-wave double dissociation was not consistent in OpenNeuro ds004696 (6 mA; automatic labels; 15–500 ms response size)

Ojeda Valencia et al. (2023) reported that hippocampal (HC) stimulation evokes a posterior cingulate (PCC) HAP-wave and that amygdala (AMY) stimulation evokes an anterior cingulate (ACC) response. We tested the predicted double dissociation in OpenNeuro ds004696 v1.0.1 using a pre-registered analysis of 6.0 mA pulses and the log10 ratio of mean-trace RMS at 15–500 ms to baseline RMS. The predicted PCC contrast (HC > AMY) occurred in 2 of 4 patients (mean +0.07; one-sided sign-flip p = 0.375); the predicted ACC contrast (AMY > HC) occurred in 3 of 5 (mean +0.16; p = 0.19). The interaction was positive in 3 of 3 (mean +0.29; p = 0.125, the smallest possible value). No within-patient test had Holm-adjusted p < 0.05 (smallest 0.051). Sub-01 showed an HC-specific PCC wave, but the sample did not show a consistent double dissociation. This is a failure to replicate the pattern in this sample, not proof that it is absent. The sample is small; region labels are automatic, the recording reference is not bipolar, and the RMS measure does not capture waveform shape or polarity. The package includes the pre-registered plan, analysis code, results, and provenance.

HAP-wave · stereo-EEG · electrical stimulation · hippocampus · amygdala · cingulate cortex · double dissociation · replication · OpenNeuro