Two-way excitatory links had an adjusted voltage response ratio of 1.29, with a 0.98–1.69 resample range, in 174 mouse V1 connections

We tested whether detected two-way excitatory connections have larger voltage responses than one-way connections in mouse primary visual cortex. We read all 123,506 pair records in Allen Synaptic Physiology r2.1. The main tested graph had 2,721 cell pairs. Both directions had a connection call and more than 10 test spikes. The voltage sample had 174 directed connections, with 50 from two-way pairs. Controls included cell-group pair, distance, age, solution, project, and both spike counts. The adjusted voltage response ratio was 1.29. Its central 95 percent mouse-group resample range was 0.98 to 1.69. This range did not clearly separate no difference from a moderate increase. In 177 release-model fits, the adjusted site-count ratio was 1.02, with a range of 0.67 to 1.56. Fifty-nine fits reached the largest allowed site count, 32. The data gave no clear account of the voltage difference in terms of fitted sites or release probability. Detected links are assay outcomes. Fitted sites are not measured anatomical synapses. Slice cuts, missed weak links, incomplete cell labels, and uncertain model parameters limit the result. A separate SQL selection and regression fit reproduced the main coefficient and cluster error. The package has code, source hashes, aggregate results, all resample coefficients, figures, and repeat steps. Original Allen records stay external.

mouse V1 · reciprocal connections · synaptic physiology · quantal release · structure and function · reproducible benchmark