Matched Allen slice data do not resolve a link between reciprocal wiring and 50 Hz measurement coverage

We tested whether reciprocal chemical wiring marks the availability of scalar 50 Hz response-change estimates in Allen Synaptic Physiology r2.1. We compared detected connections within the same recording and source and target cell-label groups. Mouse visual cortex retained 423 directed edges from 101 inferred donors. The reciprocal minus one-way coverage difference was 1.31 percentage points, with a nominal 97.5% donor bootstrap interval from -9.30 to 11.68 points. Human temporal cortex retained 27 edges from five inferred donors. Its difference was 10.00 points, with an interval from -30.00 to 60.00 points. A higher test-spike threshold and distance bins also gave primary intervals that included zero. Matching retained only 23.1% of selected mouse edges and 17.5% of selected human edges. These data do not resolve a coverage difference and do not establish random missing data. Poor overlap, slice selection, uncertain connection calls, inferred donor labels, and sparse human data limit inference. Code, edge records, an independent aggregation check, a figure, and source hashes support inspection and reproduction.

Allen Institute · reciprocal connectivity · measurement coverage · missing data · synaptic plasticity · null result