Cell-label adjustment leaves late plasticity coordination uncertain in selected Allen mouse reciprocal E-I pairs

We tested whether short-term response changes are coordinated between the two directions of reciprocal excitatory-inhibitory connections. Allen Synaptic Physiology r2.1 supplied 175 mouse dyads with dynamics QC in both directions. Late changes at 50 Hz were available in 159 dyads. Their pooled rank correlation was 0.302, with a nominal 95% inferred-donor bootstrap interval of 0.166 to 0.421. In the same sample, cell-label adjustment gave correlation 0.090, with interval -0.052 to 0.237. Fixing recording, inhibitory Cre label, and excitatory target layer left 28 dyads from 13 inferred donors. The residual rank correlation was 0.068, with interval -0.339 to 0.456. The matched result does not resolve coordination. This exploratory analysis supplies a paired evidence table and recording controls. Sparse matched support, selection, uncertain voltage fits, and inferred donors limit inference. It does not establish independence, a release mechanism, or intact-brain dynamics.

Allen Institute · reciprocal circuits · short-term plasticity · recording controls · exploratory analysis