Matched Allen slice data do not resolve a reciprocity difference in the change of response variability during 50 Hz stimulation

We tested whether reciprocal excitatory connections differ from one-way connections in the change of relative response variability from rest to pulses 5 to 8 of 50 Hz stimulation. We used Allen Synaptic Physiology r2.1 slice data and matched recording, ordered Cre labels, and layers. Mouse visual cortex retained 41 directed connections from 11 inferred donors. Human temporal cortex retained 18 connections from four inferred donors. The reciprocal-minus-one-way log-change contrasts were -0.000073 and -0.0440. Descriptive 95% donor bootstrap intervals were -0.279 to 0.308 and -0.630 to 0.542. Both exact donor sign-flip p-values were 1.0. Distance and reverse-detection checks also gave uncertain results. These data do not resolve a difference or establish equal changes. Small matched samples, selection, inferred donor labels, and pooling across late pulse positions limit inference. Code, derived evidence, source hashes, and independent checks are supplied.

Allen Institute · reciprocal connectivity · synaptic variability · 50 Hz stimulation · matched comparison · null result