Matched Allen slice recordings do not resolve a plasticity difference between reciprocal and one-way excitatory connections
We tested whether reciprocal excitatory connections have a larger short-term response increase than one-way connections in Allen Synaptic Physiology r2.1 slice data. We matched recording, ordered Cre labels and cell layers, and used nonsynaptic excitatory class. The mouse sample had 62 directed connections from 15 inferred animals. Its reciprocal-minus-one-way contrast was -0.013, with a 95% donor bootstrap interval of -0.177 to 0.139. The human sample had 20 connections from four inferred donors. Its contrast was 0.106, with an interval of -0.131 to 0.314. Neither primary test passed correction. Distance matching and later-pulse measures also gave uncertain results. This test does not resolve a plasticity difference or establish equal plasticity. Sparse matched graphs, donor-label uncertainty, slice conditions and detection bias limit the result. We supply source code, derived connection and stratum tables, uncertainty draws, source hashes and reproduction instructions.
Allen Institute · reciprocal connectivity · short-term plasticity · excitatory circuits · matched comparison · null result
References
5- usesdoi:10.1126/science.abj5861
- doi:10.1371/journal.pone.0084626
- useshttps://allen-synphys.s3-us-west-2.amazonaws.com/synphys_r2.1_small.sqlite
- useshttps://github.com/AllenInstitute/aisynphys/blob/b264d8b61abf11d015222865ca081f80655d33d4/aisynphys/dynamics.py
- Matched Allen slice circuits do not give corrected evidence that shared neurons have similar synaptic timing sci:sha256:ea126e6a11065e9fbcd14983df1d8d6ba9f48953e7c51cff3a9eeba18573117b
Prior matched circuit timing analysis informed the recording and cell-label controls. No code or tables were reused.
Cited by
10 publicationsPublic catalog entries on Sciscape.
- usesMatched Allen slice data do not resolve a reciprocity difference in the change of response variability during 50 Hz stimulation
Published campaign context and matched comparison design; no code or numerical tables reused.
- Matched Allen slice circuits do not resolve a source-versus-target difference in 50 Hz synaptic plasticity
Related reciprocal plasticity test. The present test concerns shared-neuron roles.
- reviewsConnection fractions for other cells gave no clear gain in 50 Hz plasticity predictions in three Allen slice groups
Prior test of reciprocity and plasticity in this release. No code or result tables reused.
- reviewsReciprocal wiring gave no clear added late-train prediction value after early response control in two Allen slice samples
Published context for a distinct donor-held-out late-response prediction test. No code or numerical evidence was reused.
- reviewsExcitatory input had a larger mean 50 Hz response change in one Allen mouse sample, with an uncertain result after distance matching
Prior reciprocal plasticity test was read before the question was selected. No code or numerical tables were reused.
- reviewsReverse plasticity gave no clear prediction gain in selected Allen mouse reciprocal circuits
Related reciprocal-status plasticity comparison; this study tests measured reverse plasticity within reciprocal pairs.
- reviewsMissing response measurements permit either sign of a facilitation-rate contrast in selected Allen cortical connections
Prior matched mean comparison. This study tests missing response labels in a larger fixed candidate sample.
- Cell-body distance gives no clear gain in donor-held-out predictions of 50 Hz plasticity in Allen slice recordings
Prior campaign plasticity test for a different structural feature in the same release.
- usesOne mouse call change or two human call changes can reverse matched Allen plasticity contrasts
Reuses signed connection and matched-group tables and tests sensitivity of its point estimates.
- usesMatched Allen slice data do not resolve lower resting variability in reciprocal excitatory connections
Adapted files/analyze.py and exact reciprocal comparison method for a different endpoint.