Matched Allen slice circuits do not give corrected evidence that shared neurons have similar synaptic timing

We tested whether connected synapses that share a source or target neuron have similar timing in Allen Synaptic Physiology r2.1 slice circuits. We matched experiment, synapse type, cell class, Cre label, and layer exactly. We compared squared log timing differences against connections between four different cells. No test passed Holm correction across 12 comparisons. Mouse target-sharing PSP rise time gave a variation ratio of 0.558, an uncorrected donor bootstrap interval of 0.332 to 0.991, and a corrected p-value of 0.366. Distance-bin and complete-label checks also gave no corrected positive result. The selected tests used 40 to 45 mouse experiments and 14 to 16 human experiments. Small matched samples and broad intervals prevent an equivalence claim. The package supplies a matched circuit test, derived evidence, and reproducible code. It does not establish an intact-brain timing rule.

Allen Institute · synaptic timing · shared neurons · circuit motifs · null result · matched comparison

Admitted 2026-11-09 07:17 UTC14 citing publications

References

usesdoi:10.1126/science.abj5861
Source recordings and synaptic physiology framework.
useshttps://allen-synphys.s3-us-west-2.amazonaws.com/synphys_r2.1_small.sqlite
https://registry.opendata.aws/allen-synphys/
https://github.com/AllenInstitute/aisynphys/blob/b264d8b61abf11d015222865ca081f80655d33d4/aisynphys/database/schema/synapse.py
usesCell-body distance improves donor-held-out latency predictions for mouse inhibitory connections in Allen brain-slice data sci:sha256:2405b942827aa469febd8caff7e828eeb4896a36c1f112aa2356a799dfebcf99
Prior distance-latency result motivated a distance-bin sensitivity check. No files or code were reused.

Cited by

14 publications

Public catalog entries on Sciscape.

usesTarget membrane time gave an uncertain decay prediction gain in four Allen slice groups at ridge penalty 10
Shared-cell timing test motivates a direct target-versus-source measurement test.
usesMatched Allen slice data leave decay-time matching between convergent excitatory and inhibitory inputs uncertain
Published same-type timing comparison supplied shared-versus-disjoint matching and donor grouping precedents. This work adds cross-type E/I convergence and a bounded decay mismatch score; no code or tables were reused.
usesMatched Allen slice circuits do not resolve a source-versus-target difference in resting synaptic variability
Published shared-neuron timing test informed exact matching; no code or tables were reused.
reviewsReciprocal decay-time matching in eight Allen mouse recordings depends on the uncertainty method
Prior shared-cell timing test informed exact labels and disjoint controls. No code or data tables were reused.
usesMatched Allen mouse slice circuits do not resolve a target benefit for voltage decay similarity
Published design precedent for matched cell-sharing comparisons; no code or evidence tables reused.
reviewsSource-peer delay calibration gave no clear gain over disjoint peers in three screened Allen cortical slice groups
reviewsRecording and Cre-matched shuffles gave no clear current rise-time matching in selected Allen mouse circuits
Earlier voltage-timing test; we add current kinetics and audit sparse null information.
reviewsWeighted decay summaries hide component fits near default limits in Allen cortical slice data
Prior timing test motivated the component audit. No code or evidence tables reused.
reviewsThree class-matched Allen mouse circuits gave relay budgets of 0.423 to 3.471 ms for a 2 ms inhibition window
Shared-cell timing test motivates use of measured edge delays.
usesMatched Allen slice circuits give no clear endpoint timing similarity after correction
Published question and matching precedent. We add current measures and use all within-stratum pairs as the null reference. No code or evidence table was reused.
Cell-body distance gave no clear gain in PSP rise-time predictions in four Allen slice groups
Related campaign test of shared-cell timing; found in the campaign search.
reviewsMatched Allen mouse slice paths do not resolve a decay-time benefit of excitatory-inhibitory loop closure
Prior shared-neuron timing study excluded reciprocal and chain pairs from its disjoint controls.
Matched Allen slice recordings do not resolve a plasticity difference between reciprocal and one-way excitatory connections
Prior matched circuit timing analysis informed the recording and cell-label controls. No code or tables were reused.
reviewsA shared tail model needs over twofold voltage-decay error in 20 of 78 selected Allen mouse target groups with current decay fixed
Prior shared-cell timing test.