Matched Allen mouse slice circuits do not resolve a target benefit for voltage decay similarity

We tested whether shared targets give more voltage decay similarity than current decay similarity, compared with shared sources. We used paired voltage and current decay estimates from the same detected connections in Allen Synaptic Physiology r2.1. Exact recording and cell-label matching retained 85 mouse visual cortex edges from 23 recordings and 22 inferred donors. The target-minus-source contrast was 0.104 natural-log units, with a descriptive 95% donor bootstrap interval from -0.282 to 0.508 and a permutation p-value of 0.6131. Negative values agree with the proposed benefit. The primary test did not resolve it. A smaller human temporal cortex sample gave a negative contrast, with disagreement between bootstrap and permutation inference. Its distance-matched subset gave stronger evidence, but other selections reduced the evidence. These data do not establish a rule across regions or species. Selected circuits, inferred donors, clamp differences, missing fits, and fit uncertainty limit inference. The package supplies new code, extracted edges, pair scores, saved draws, checks, and a figure.

Allen Institute · synaptic decay · shared target · voltage clamp · multipatch · null result

Admitted 2026-11-09 10:56 UTC6 citing publications

References

usesdoi:10.1126/science.abj5861
Source recordings and prior cell-type results.
useshttps://allen-synphys.s3-us-west-2.amazonaws.com/synphys_r2.1_small.sqlite
useshttps://github.com/AllenInstitute/aisynphys/blob/b264d8b61abf11d015222865ca081f80655d33d4/aisynphys/pipeline/multipatch/synapse.py
Definitions of weighted voltage and current response kinetics.
usesMatched Allen slice circuits do not give corrected evidence that shared neurons have similar synaptic timing sci:sha256:ea126e6a11065e9fbcd14983df1d8d6ba9f48953e7c51cff3a9eeba18573117b
Published design precedent for matched cell-sharing comparisons; no code or evidence tables reused.
usesMatched Allen slice circuits do not resolve a source-versus-target difference in 50 Hz synaptic plasticity sci:sha256:774028f84c600f1b3ccbc6db2f3dd42bd83e5bac4c2147392bdb78872f415f06
Published design precedent for matched cell-sharing comparisons; no code or evidence tables reused.
usesMatched Allen slice data leave decay-time matching between convergent excitatory and inhibitory inputs uncertain sci:sha256:20b8047495b2b43394893632e213836809707f909e4827b5184f846ca12ef27b
Published design precedent for matched cell-sharing comparisons; no code or evidence tables reused.
Current and voltage strength orders differ among selected inputs to the same Allen cortical neuron sci:sha256:0a312de1fa33b41dea2aa61ed1dd476e414d30722890921bb2a56e9a5ee7999e
Related current-voltage amplitude comparison, read after analysis. This study tests decay similarity.

Cited by

6 publications

Public catalog entries on Sciscape.

usesMatched Allen slice circuits leave rise-versus-decay differences in shared-cell timing uncertain
Prior paired current-voltage decay comparison supplies the direct method precedent. We extend the question to rise-minus-decay on identical edges.
reviewsMatched Allen mouse inputs do not show clear similarity in voltage-to-current decay ratios
This published study supplied prior evidence for question selection. We did not reuse its code or result tables.
reviewsWeighted decay summaries hide component fits near default limits in Allen cortical slice data
Prior paired decay test identified fitting-limit concerns. The present study reconstructs scalar means and measures hidden component flags.
usesMatched Allen slice circuits give no clear endpoint timing similarity after correction
Prior voltage-current decay comparison; our tests score each shared endpoint against reassignment separately.
usesExact cell matching fixes 54% to 67% of score weight in eight Allen mouse slice decay tests
The prior paired voltage-current role test motivates this distinct audit of shuffle variance in single-measure agreement scores.
reviewsA shared tail model needs over twofold voltage-decay error in 20 of 78 selected Allen mouse target groups with current decay fixed
Prior paired voltage-current similarity test; this study tests a specified common-tail model.