Matched Allen slice circuits leave rise-versus-decay differences in shared-cell timing uncertain

We tested whether the source-versus-target contrast in paired voltage and current timing differs between response rise and decay. Exact cell-label and recording matching in Allen Synaptic Physiology r2.1 retained the same connections and motifs for both features. The main mouse inhibitory sample had 50 connections, 13 recordings, and 13 inferred donors. Rise contrast minus decay contrast was 0.112 natural-log units, with a descriptive 95% donor bootstrap interval from -0.479 to 0.703. Mouse excitatory and human excitatory intervals also included zero. Only one human inhibitory donor remained. Removal of decay values near fit endpoints left at most three donors per sample. These selected circuits do not resolve a timing-scale difference. This exploratory extension of prior decay work supplies a paired endpoint, exact evidence tables, source code, and independent selection and point-estimate checks. Inferred donor groups, small samples, missing fits, different clamp states, and fit uncertainty limit inference. The result does not establish equivalence, a membrane mechanism, or transfer across regions or behavior.

Allen Institute · synaptic kinetics · shared neurons · rise time · decay time · null result