Matched Allen slice data leave a reciprocity difference in successive-response correlation uncertain

We tested whether reciprocal excitatory wiring identifies a difference in correlation between the first two responses to 50 Hz stimulation. We used Allen Synaptic Physiology r2.1 and matched recording, ordered cell labels and layers. Mouse visual cortex retained 58 connections from 14 inferred donors. Human temporal cortex retained 22 connections from four inferred donors. Reciprocal-minus-one-way Fisher z contrasts were -0.0387 and -0.0616. Descriptive 95% donor bootstrap intervals were -0.2455 to 0.1814 and -0.2172 to 0.1177. Both Holm-adjusted exact donor sign-flip p-values were 1.0. Raw-correlation, reverse-detection and distance checks also left differences uncertain. The data do not establish equal response statistics. Small matched samples, missing outcomes, uncertain reverse calls, inferred donors and unequal unknown trial counts limit inference. Original code, aggregate evidence, a figure and external-data retrieval instructions support reproduction. External records are excluded.

Allen Institute · reciprocal connectivity · successive-response correlation · 50 Hz · synaptic dynamics · null result