With 0.5 s filters, the second calcium match missed the closest spike rival in 30 of 40 blocks from 20 rat spine records
We tested a step used to map recorded spike sources to imaged dendritic spines. The second calcium match need not be the source most similar to the selected spike trace. With 0.5 s filters and fixed signal controls, this mismatch occurred in 30 of 40 blocks. The data came from 20 spine records in 19 recordings from 10 rat cortical cultures. A full release model with no added noise gave 14 changed scalar decisions when all rivals were checked. At a release success probability of 0.5, another rival gave a larger 95th percentile in 28 blocks. Fifteen scalar decisions changed; Monte Carlo error bounds kept 14 changes. An exact example proves that the two candidate orders can differ. These conditional signal tests do not estimate anatomical errors or repeat the source paper's full acceptance method. We provide all candidate scores, source arrays, simulation draws, code, figures, and repeat steps. A new source extraction matched all 209 arrays. Independent signal checks and a clean offline repeat passed.
functional connectivity · dendritic spine · source identification · calcium imaging · spike trains · competing source · release failure · correlation
References
4- challengesdoi:10.1088/1741-2552/ac8765
We test the reduction of alternative-source checks to the second calcium match. Our exact example and defined signal audit show that the two source orders can differ. We do not estimate the error rate of the complete published method.
- usesdoi:10.48324/dandi.000223/0.260528.0906
This fixed release supplies all fluorescence, camera times, unit IDs, and spike times for 19 distinct recordings and 20 spine records.
- usesWith 0.5 s filters, a past spike rule reduced calcium test error by 30% after shaft and population controls in 20 spines from 10 rat cultures sci:sha256:6de11b8c382803c76f8bff29db200d2f4eea777c716b92afe5ab5eed75c79b4f
We reuse its verified source arrays, provenance, MIT event-sum and baseline operations, and MIT extraction code. We add the complete rival audit and release checks.
- supportsWith 0.5 s filters and ridge peer controls, a selected unit gave an uncertain 7.4% calcium prediction gain in 20 rat spines sci:sha256:16b12b0abba9d8fe33c016eb31113db125d9a28f9ff59d51744223279e2faa15
The rival audit gives separate evidence that a useful calcium match does not identify a unique spike source. It tests a different inference step from ridge prediction gain.
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