Movie response similarity had a stronger link to connection rate for layer 5 than layer 2/3 targets in one MICrONS mouse

We tested a reported layer trend with a direct joint-model contrast in public matched activity and electron microscopy data from one MICrONS mouse. We selected 281,542 candidate pairs with measured movie response correlations and layer labels. A Poisson model used source-by-target-layer effects, target-cell effects, layer-specific proximity terms, and clustering by both cells. It retained 151,676 informative pairs. Rate ratios per common response-correlation SD were 1.0650 for layer 2/3 and 1.1759 for layer 5. Their ratio was 1.1041, with a nominal 95% interval of 1.0440 to 1.1676. Distance and correlation-range checks gave similar results. The higher-visual-area source subset remained uncertain. This is a follow-up to published subgroup patterns, not independent confirmation. One animal, selected cells, separation, layer-dependent measurement error, and model assumptions limit inference. The result does not establish causal computation or a rule across animals. Code, scalar evidence, covariance matrices, source hashes, numerical checks, and a figure support reproduction.

MICrONS · layer-specific connectivity · measured movie responses · structure and function · joint slope contrast