Separate cell gains fit all 14 selected direct-wiring response magnitude ratios between normal and unc-31 worms

We tested whether separate gains for stimulated and responding cells can fit response changes between normal and unc-31 worms. We linked the Randi calcium-response atlas to two adult Witvliet wiring maps. Fourteen directions had direct wiring in either map and detected responses in both strains. Their observation graph had 26 role nodes, 12 components and no independent cycles. Thus every response magnitude ratio has an exact cell-gain fit. One direction changed sign; it had one mutant occurrence and was removed by a two-occurrence threshold. All 24 direct-wiring sensitivity selections had no cycles. A wider sample of 43 directions had one six-edge cycle and a minimum multiplicative ratio tolerance of 1.538183. No measured error bound supports biological rejection. Separate animals, selected pooled estimates and free role gains limit inference. We provide proof, code, source hashes, paired evidence and numerical checks.

C. elegans · unc-31 · structure and function · identifiability · cell gains · observation design