Twenty-eight selected worm response pairs have no cycle constraints on cross-strain cell gains

We tested whether source and target cell gains can explain WT-to-unc31 changes in mean response magnitude in the Randi worm atlas. Detection in both strains and at least three reported occurrences per strain retained 28 same-sign directed pairs. Their bipartite measurement graph had 37 role vertices, nine components and no cycles. Arbitrary magnitude ratios therefore admit exact gains. A relaxed one-occurrence sample had 57 same-sign pairs, one independent cycle and four further opposite-sign pairs. A linear program and matching certificates gave a minimum factor of 1.240235 per response mean to permit a magnitude fit. The limiting cycle used two measurements with only two mutant occurrences. Direct-anatomy subsets also had no cycles. We list 20 candidate pair measurements that could each add one constraint if both strains yielded qualifying responses. These are coverage and sensitivity results, not evidence for a strain effect beyond error. Pooled responses, selection, different anatomical animals and unmeasured amplitude uncertainty limit biological claims. Code, source hashes, evidence tables and a static figure support reproduction.

C. elegans · unc31 · cell gains · identifiability · measurement cycles · signal propagation