Four tests did not confirm more similar 50 Hz response changes on selected mouse V1 inhibitory connections that share a cell

We tested whether inhibitory connections that share a source or target cell have more similar 50 Hz response changes. We used Allen Synaptic Physiology r2.1 small, mouse V1, and separate scalar initial and induction measures. We held recording, source and target markers, nonsynaptic cell classes, and intended layers fixed. Of 785 available directed connections, 153 from 37 inferred animals entered at least one graph test. Each role used 32 recordings. We compared shared-role squared differences with all edge-pair differences inside each group. We tested four mean scores with 19,999 within-group label assignments and Holm correction. Source scores were 0.215 initially and 0.194 at pulses six through eight; adjusted p-values were 0.197 and 0.203. Target scores were -0.232 and -0.157. No main test passed correction. Pointwise animal intervals still permit positive source effects. In a secondary prediction test, a fixed half-weight correction from other outputs of the same source reduced mean error by 12.4% initially and 3.6% at pulses six through eight. Both intervals included zero. A correction from other inputs to the same target increased mean error by 27.6% and 18.8%. The initial target result was sensitive to one recording. Pvalb subset and rank checks gave positive source leads but are not independent replications. The results leave a source relation plausible and give no clear source prediction gain under the tested rule. Sparse groups, response availability, coarse labels, shared measurement conditions, and unvalidated waveform fits limit biological inference. The package includes independent code, connection keys, aggregate results, figures, source hashes, validation, and repeat instructions. Original Allen data and software remain external.

inhibitory circuits · short-term plasticity · structure and function · shared source · shared target · conditional permutation · mouse visual cortex · synaptic physiology