Electrical calls marked lower coverage of 50 Hz chemical response data in Allen r2.1 mouse V1 Pvalb pairs

We tested whether stored chemical response dynamics preserve the electrical link composition of a selected cortical circuit sample. We used Allen Synaptic Physiology r2.1 small. The sample had 509 directed inhibitory chemical links between mouse V1 cells with Pvalb labels, from 399 cell pairs and 208 inferred animals. A positive electrical call in either direction marked a pair positive. A quality-passed finite 50 Hz induction measure was available for 16 of 96 electrical-positive links and 275 of 413 other links. Electrical-positive links formed 18.9% of all selected links but 5.5% of links with dynamics. The coverage risk ratio was 0.250, with a whole-animal 95% resampling range of 0.139 to 0.383. Lower coverage appeared in all three projects and after electrical-call agreement and cell-pair checks. A fit within recordings was sparse: only 13 recordings varied in both call and availability, and one animal deletion caused separation. All 16 available electrical-positive induction values were negative, versus 264 of 275 others. With observed signs fixed and unavailable signs unrestricted, the full-set negative-fraction difference ranged from -80.7 to +36.1 percentage points. Thus, the available signs do not identify the group order in all selected links. These are data-coverage findings, not effects of electrical coupling on chemical transmission. We supply original code, aggregate results, figures, exact source versions and hashes, validation, and repeat instructions. Original Allen data and software remain external under their source terms.

electrical synapses · chemical synapses · short-term plasticity · Pvalb · mouse visual cortex · data selection · missing data · functional connectivity