With recording and log distance controls, manual electrical calls marked more chemical links in 1,471 mouse V1 Pvalb pairs

We tested whether electrical and chemical connection calls occur together in local maps of mouse visual cortex. We used Allen Synaptic Physiology r2.1 small and counted each neuron pair once. Both cells had Pvalb labels and nonsynaptic inhibitory labels. A call in either direction made a pair positive. The selected set had 1,471 pairs from 575 recordings and 344 inferred animal groups. Chemical calls occurred in 65 of 100 electrically positive pairs and 326 of 1,371 other pairs. A conditional logistic model controlled for recording group and log soma distance. The electrical odds ratio was 3.37, with a 95% animal-cluster interval from 1.21 to 9.39. The fit used 640 pairs in 119 informative recordings. Only 38 recordings varied in both call types. Removing each animal group in turn kept the odds ratio from 3.05 to 5.38. Requiring agreement between the two electrical calls gave 2.57 (0.85 to 7.73). Requiring numerical pulse summaries in both directions gave 1.67 (0.31 to 9.14). These wide intervals overlap the primary result. The evidence supports a manual-call association in the broad selected set. It does not establish a positive association under every data requirement. Shared review errors, cell differences, and slice selection remain possible explanations. We supply original code, all computed model checks, source hashes, and repeat instructions. The code recreates a 4,705-pair extract across three cell labels. The original database and this row-level extract stay external.

electrical coupling · chemical synapse · Pvalb · mouse visual cortex · conditional logistic regression · paired recordings · Allen Synaptic Physiology