In 126 mouse layer 5 recordings, a log model gave a prefrontal versus sensory delay SD ratio of 1.28 after response controls

We tested whether cortical region adds information about synaptic timing after response size and failure rate are known. We used released paired recordings between layer 5 pyramidal neurons in mouse prefrontal and primary somatosensory cortex. The main sample had 126 recordings from 85 dates. A common-slope model used log delay standard deviation (SD), log current size, failure fraction, and its square. The raw geometric mean regional ratio was 2.50. The adjusted ratio was 1.28, with a 95% date resampling range of 1.05 to 1.54. The regional log coefficient fell by 73%. All specified checks retained a positive residual ratio. Each prediction test excluded a full date. Adding region after the controls gave 3.73% less test error. A later date bootstrap with complete refits gave a gain range from -7.25% to +15.91%. Thus, a conditional regional association remained, but the added prediction gain was uncertain. A raw source-sheet audit avoided an offset in the released summary columns. A numerical rounding check retained all 127 source recordings and gave a ratio of 1.279. Region was fully confounded with collection era, and verified animal IDs were absent. The result does not identify calcium channel distance or separate biological timing from onset-fit error. The package contains the source workbook, a checked table, the saved plan, all predictions, resampling draws, figures, new code, and offline repeat instructions.

synaptic timing · prefrontal cortex · somatosensory cortex · structure and function · release reliability · calcium coupling · paired recording · reproducible analysis