A twofold cell gain-ratio bound requires a response change factor of 1.415 in 36 selected worm pairs

We tested a reciprocal transfer model with bounded cell gain ratios in selected C. elegans responses. We linked the Randi WT atlas to two adult Witvliet anatomy maps. In 36 same-sign pairs with a direct link in either adult, q below 0.05 and at least three occurrences per direction, a twofold gain-ratio range requires a minimum per-response change factor of 1.414608. Unrestricted gains require factor 1.081936, reached at a gain-ratio range of about 11.606842. The 22-pair subset with a link in both adults has no cycles. Exact fits in that subset need gain-ratio range 25.511236; a twofold bound again needs response factor 1.414608. Stricter selections reduce the required gain ranges. Linear programs, dual checks and independent difference-constraint checks support the results. No gain or amplitude error bounds were measured. This is a conditional model sensitivity test, not evidence of biological nonreciprocity. Different animals, selected coverage, pooled calcium means and separable gain assumptions limit interpretation. Original code, aggregate results, a figure and pinned external data retrieval steps support reproduction.

C. elegans · connectome · reciprocity · calibration · cell gains · bounded error