Frequency changes input order in 90 of 423 selected Allen fit comparisons under a fixed-efficacy model

We tested whether frequency changes the strength order of two same-sign inputs to one cell in Allen cortical slice response fits. A linear model with a finite rise and fixed synaptic efficacy changed the magnitude order between 1 and 40 Hz in 90 of 423 matched fit comparisons from 393 input pairs. Nested donor means were 15.11% for mouse excitation, 26.14% for mouse inhibition and 28.47% for human excitation. Human inhibition had no reversal in ten comparisons from two inferred donors. Only 23 comparisons retained reversal under all amplitude changes by factors from 1/1.25 to 1.25 with timing fixed. These error factors are assumptions. The model omits short-term plasticity and does not measure responses to frequency modulation. Selected slice circuits, uncertain fits and inferred donor labels limit biological inference. Code, aggregate evidence, a figure and external-data retrieval instructions support reproduction.

Allen Institute · convergent inputs · synaptic filtering · frequency response · amplitude uncertainty · linear model