Voltage and current measurements change a factor-of-two strength rule in 11 of 28 selected Allen reciprocal excitatory pairs

We compared reciprocal excitatory strength ratios across recording modes in Allen Synaptic Physiology r2.1 slice data. Of 48 eligible pairs, 18 mouse visual cortex pairs and ten human temporal cortex pairs had four usable amplitudes and passed fit filters. A factor-of-two matching rule changed between voltage and current responses in seven mouse pairs and four human pairs. The larger-response direction agreed in 24 of 28 pairs. Equal-donor mean differences in absolute log ratios were 0.1490 in mouse and 0.0152 in human. Descriptive 95% donor bootstrap intervals were -0.2157 to 0.4701 and -0.4327 to 0.4491. Both primary tests remain uncertain. A minimum-amplitude check changed the mouse result. These counts show that the rule can depend on recording mode in this selected sample. They are not population rates or conductance estimates. Missing measurements, inferred donors, slice selection and unmodeled fit error limit inference. The package gives code, all eligible pair records, source hashes, uncertainty draws, a figure and an independent checker.

reciprocal connectivity · synaptic strength · Allen Institute · recording mode · paired measurements