At a cutoff of five synapses per edge, ACh/GABA three-neuron cycles in full FlyWire v783 had higher GABA presence but lower odd-GABA share than region sample means with reciprocal links fixed

We tested transmitter labels in induced directed three-neuron cycles in one female FlyWire v783 brain. The main cutoff was five synapses per directed connection. Every sampled control kept all measured reciprocal links fixed. Other fixed counts were each neuron's partner totals, partner counts by transmitter, or those counts by transmitter and main region. Among 123,468 cycles with only ACh or GABA labels, 90.46% had at least one GABA neuron. The region-control mean was 83.99%, with a sampled range of 83.66 to 84.37%. The measured odd-GABA share was 54.00%. It was above the total-count control mean of 50.22%, but below the transmitter-control mean of 60.00% and the region-control mean of 55.12%. At ten synapses, GABA presence stayed above the sampled range. The odd share lay within it. Thus GABA presence and odd-GABA cycle shares gave different comparisons. Only the odd share gives a negative loop product under an added binary sign model. We did not measure physiological signs or activity. Results describe finite switch references from one brain. An independent counter matched every measured transmitter triple at both cutoffs. Code, source hashes, complete traces, and exact small sampler checks are supplied.

connectome · Drosophila · FlyWire · GABA · directed cycles · network motifs · conditional graph controls · reciprocity