A decreasing frequency order needs changes above 0.10 in 173 of 358 selected Allen connections

We tested a necessary frequency-order condition for a one-pool depletion model with constant release and full initial recovery. We used protocol-specific response changes at 10, 20, and 50 Hz in Allen Synaptic Physiology r2.1. The sample had 358 detected connections with at least five trials per frequency. An exact uniform-error bound showed that 173 need changes greater than 0.10 normalized units to permit a decreasing order. These include 70 of 134 mouse excitatory, 90 of 183 mouse inhibitory, 11 of 38 human excitatory, and two of three human inhibitory connections. The bound is an assumed tolerance, not measured uncertainty. A six-trial minimum left only 16 connections. The test screens a restricted temporal model; it does not reject depletion as a mechanism or show a general rule across regions. Code, aggregate findings, proof, figure, hashes, and external retrieval instructions support reproduction. Source records stay outside the bundle.

Allen Institute · short-term plasticity · frequency order · depletion model · error bound · cortical circuits