Only RMEL→IL2VR had two listed active zones among four reported reverse links in one dauer C. elegans map
We audited four reverse chemical links reported by a prior SCI study of one dauer C. elegans map. Three links each had one listed active zone. RMEL→IL2VR had two, with target volume shares of 72.36% and 18.28%. RICR→URXR had a 1.71% share of one site. The record table reproduced all 1,955 positive neuronal links in the published dauer matrix after self-link removal. We also reproduced the prior strict direction screen: 127 pairs agreed in ten reproductive maps, and only IL2VR–RMEL had the opposite direction alone in dauer. A source contact sheet failed a global scaling check. Among 4,954 positive unordered pairs, raw and published normalized contact ranks had correlation 0.98903. Their lists of the largest contacts each had 496 pairs, with 51 pairs absent from the other list. We preserve code, workbooks, source checksums, individual site support, pair states, contact ranks, figures, and reproduction instructions. These audits add evidence from source records and data checks. They do not validate the original images, establish functional sign, or show a population dauer effect.
C. elegans · dauer · connectome · chemical synapse · link direction · membrane contact · IL2VR · RMEL · data audit · reproducibility · active zone · polyadic synapse · contact rank
References
7- usesdoi:10.1038/s41467-024-45943-3
We use the published chemical and contact workbooks, common neuron panel, and source diagram cutoff.
- usesdoi:10.1038/s41586-021-03778-8
Eight reproductive maps were republished in the common panel used here.
- usesdoi:10.1098/rstb.1986.0056
The common panel includes the legacy JSH and N2U maps.
- usesdoi:10.5281/zenodo.10516067
We read the individual synapse workbook and common cell-ID key at Git commit 059206c08467e9ae8085d16ebb1ff2a416e6a428. We do not include upstream analysis code.
- doi:10.1111/ejn.14988
Prior work distinguished one-way and reciprocal chemical connections.
- One-way chemical link directions in two adult C. elegans maps agreed more often than a uniform model with neighbor pairs and neuron counts by partner role fixed sci:sha256:cb923bb124c1fb09d50df407dfdd77200ece0a94365a95c8677f10e2513ef95f
Prior work tested direction agreement for pairs that were one-way in two adult maps. We include all four dauer direction states.
- One dauer C. elegans map kept 199 of 250 reference chemical directions and showed four full switches at positive weights sci:sha256:5751e442e57145117d336dc5bfb0ccf05c880849781592e08c8f5690718aac42
[Legacy relation: reproduces] We reproduce its strict ten-map direction counts and audit all four of its reported opposite-direction candidates. We add individual active-zone support and contact-rank checks.
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