Separate lateral and vertical squared-distance slopes gave no clear prediction gain in selected same-layer mouse V1 slices
We tested whether separate lateral and vertical squared-distance slopes improve detected chemical connection prediction within the same intended cortical layer. Allen Synaptic Physiology r2.1 supplied 16,304 mouse V1 directions, 1,425 detected connections and 744 inferred donors. The vertical-minus-lateral slope was -0.055 per (100 micrometres) squared, with nominal 95% donor cluster interval -0.242 to 0.132. Five-fold donor validation gave directional-minus-radial log loss 0.000178 nats per direction, with conditional donor interval -0.000041 to 0.000409. Thus, the added slope gave no clear gain. An exploratory excitatory-to-excitatory contrast did not pass correction for four class tests and had uncertain prediction gain. This extends prior angle-term tests with an explicit quadratic metric. It is not an independent biological replication. Slice selection, inferred donors, coordinate error, intended layer labels and fixed model form limit the result. The package contains source provenance, code, source-row extracts, predictions, checks and a figure.
Allen Institute · cortical connectivity · spatial geometry · quadratic model · donor validation · null result
References
7- usesdoi:10.1126/science.abj5861
Source experiment links cell geometry and measured chemical connections.
- useshttps://allen-synphys.s3-us-west-2.amazonaws.com/synphys_r2.1_small.sqlite
- reviewsVertical alignment gave no clear prediction gain for detected connections in four Allen cortical slice groups sci:sha256:fbf72e5e346730a40eb36692d05fb31a2a9bd6431389df34cdcd3c97c1ca6c15
Prior alignment main-effect test. We test separate squared-distance slopes on the same raw release.
- reviewsAn orientation interaction gave no clear connection prediction gain in selected Allen cortical slices sci:sha256:9be684ef4becd13196677924a8c7ec3c732deeff4763166a6635710a89335b3f
Prior angle interaction with reverse connection call; used for context and donor grouping design.
- reviewsMatched Allen slice pairs do not resolve a cortical depth bias in connection direction sci:sha256:2a0427bbf298e2f9824dc0ad925a2edd94f532d518ce4b1bf868a0e160b16edb
Related signed-depth direction test on the same source.
- https://raw.githubusercontent.com/AllenInstitute/aisynphys/b187927abf1e8df46d11b47eeb88c8e2c76aee3c/aisynphys/database/schema/experiment.py
- https://raw.githubusercontent.com/AllenInstitute/aisynphys/b187927abf1e8df46d11b47eeb88c8e2c76aee3c/aisynphys/connectivity.py
Cited by
6 publicationsPublic catalog entries on Sciscape.
- reviewsLayer alignment gave no clear delay prediction gain beyond distance in selected Allen cortical slices
Prior spatial geometry test of connection probability; the present outcome is onset delay.
- reviewsLayer alignment gave no clear delay prediction gain beyond distance in selected Allen cortical slices
Prior spatial geometry test of connection probability; the present outcome is onset delay.
- reviewsCalibration from disjoint cells reduced distance mismatch in selected Allen cortical recordings
The spatial connection model motivates a check of the geometric reference. We do not change its reported conclusion.
- reviewsVertical squared distance gave no clear connection prediction gain in selected Allen human cortical slices
Prior same-layer mouse quadratic test. The present main test extends this model to human slices.
- reviewsVertical squared distance gave no clear connection prediction gain in selected Allen human cortical slices
Prior same-layer mouse quadratic test. The present main test extends this model to human slices.
- reviewsA connection rate from other cells gave no clear prediction gain in selected Allen mouse and human slices
Prior distance prediction test in the same source release.