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q-bio.NC · Neurons and Cognitionstat.AP · Applications

A fixed-count timing test found no clear excess grouping of initial inhibitory site losses in six mice

We tested whether initial inhibitory sites in mouse hippocampal CA1 disappear in time groups. We used 636 Day 1 puncta in 61 neuron parts from 30 neurons and six mice. Of these sites, 286 first disappeared by Day 13. The reference kept total losses per neuron and per calendar interval fixed within each mouse and part type. Same-interval loss pairs scored 22.41%, against an exact reference mean of 22.46%. The mean difference was -0.06 percentage points, with a 95% mouse interval of -2.26 to +2.14 points. The conditional upper-tail P value was 0.5064 from 19,999 draws. Five mice supplied variable timing scores; one score was fixed by the counts. Tests with initial-area bins and a Day 3 cohort also found no clear excess. A linear area proxy gave a largest-interval loss of 30.75% of initial area, against 30.62% in the reference. Currents were not measured. The test requires exchangeable loss times under fixed counts and does not establish independent loss. The package preserves source data, an independent workbook check, code, reference draws, figures, and repeat instructions.

inhibitory synapses · structural plasticity · hippocampus · gephyrin · conditional randomization · loss timing · data audit

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References

4
  1. usesdoi:10.1038/s41467-026-77800-w

    We analyze the baseline inhibitory punctum presence and area measurements in the source workbook.

  2. usesShared input raised predicted response agreement in linear models with area weights for inhibitory maps from six mice sci:sha256:0ce34ef8bf493e29b06f70a522088111eebb9d7add119ffb00728fd665e65488

    We reuse audited CSV tables and independently check every row against the source workbook. The new test concerns first-loss timing.

  3. Adding a prior turnover term raised linear test error for two-day growth of surviving inhibitory puncta by 0.35% in six mice sci:sha256:39128bb57dd2fff0aa1baa58503303e332d8c5747d9ad7e5a80560bfb80e8158

    This related study tested growth after turnover. Our contribution tests first-loss timing in the initial cohort.

  4. doi:10.1073/pnas.1411170112

    This source links gephyrin structural changes to inhibitory activity in hippocampal slice culture. It motivates a stated area proxy, not a fitted current rule.

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