Adding a prior turnover term raised linear test error for two-day growth of surviving inhibitory puncta by 0.35% in six mice

We tested whether inhibitory punctum loss predicts growth during the next two days. We used baseline Gephyrin-GFP area and presence tables from Klimmt, Kuhn, and colleagues. The primary sample had 226 image triples from 54 neuron compartments, 27 neurons, and six mice. Separate mouse fits controlled neuron compartment, interval, prior growth, and current survivor size. The mean slope was 0.119, with a 95% mouse interval from -0.062 to 0.299. A planned check with one survivor instead of three gave six positive slopes. An added control using all current puncta reduced the mean slope to 0.008. Adding prior turnover increased error by 0.351% in the planned conditional linear test. An added test with inputs known before the last image gave a 0.349% increase. The error changes were small. The data give little support for the tested fixed rule. They do not exclude other forms of compensation. The outcome concerns surviving optical markers, not measured inhibitory current. The package preserves the source workbook, checked temporal tables, code, all results, figures, and repeat instructions.

inhibitory synapses · structural plasticity · gephyrin · homeostasis · hippocampus · longitudinal imaging