Reference electrical weight showed no clear change in adjusted coactivity slopes between arena zones in 19 C. elegans

We tested whether the relation between electrical anatomy and calcium coactivity changes near an aversive copper boundary. We used recordings from 27 freely moving C. elegans. Nineteen animals had at least 150 usable frames in both arena zones. We removed a fitted span of measured behavior, position and time. We then fit coactivity slopes for reference electrical weight, with class identity, chemical wiring and soma distance as controls. The panel had 15,843 observations of 1,754 class pairs. Electrical slopes were positive in both zones in all 19 animals. The mean boundary-minus-interior change was 0.000514 Fisher z per feature standard deviation. Its 95% animal-bootstrap range was -0.001823 to 0.002841. A circular time-shift check gave a two-sided tail fraction of 0.770. All planned electrical sensitivity ranges also crossed zero. These fitted slopes do not measure causal coupling or prediction for new class pairs. In 500 splits into disjoint animal groups, pair coactivity patterns had median correlations of 0.820 in the interior and 0.740 near the boundary. Pair differences had a median correlation of only 0.035. Thus, state differences repeated poorly across animals, which limits this anatomy test. The result does not establish equal biological coupling between zones. Both reference electrical maps are incomplete and come from other animals. The package has exact class traces, behavior arrays, provenance, code, figures, all results and offline repeat instructions.

C. elegans · connectome · calcium imaging · electrical synapses · behavior adjustment · state changes · coactivity · repeatability · open data