Contact count left a change in the movie-related cleft-size slope uncertain in one MICrONS mouse

We tested whether the relation between measured movie response similarity and mean synaptic cleft size differs between one-contact and multiple-contact connections. We used 8,128 connected pairs from the public MICrONS functional connectomics v1 table. Separate source and target cell effects left a core of 5,158 pairs, including 531 multiple-contact pairs. The model also controlled contact count and axon and dendrite proximity lengths. The slope difference was -0.066 log-size units per standard deviation of movie correlation. Its nominal shared-neuron 95% interval was -0.147 to 0.015. Complete source-deletion refits gave an interval of -0.177 to 0.045. Clipped size, arithmetic size and a one-or-two-contact restriction also left the difference uncertain. A same-scan check retained only 28 pairs after cell controls. These results do not establish equal slopes or reject published marginal size-function relations. One mouse, selected connections, different stratum support, response error and approximate conditional uncertainty limit the test. Code, scalar data, source hashes, deletion fits and numerical checks support reproduction.

MICrONS · Allen Institute · contact multiplicity · cleft size · response similarity · cell effects