Measured movie response similarity is linked to multiple contacts after cell controls in one MICrONS mouse
We tested whether response similarity is linked to multiple synaptic contacts among connected neuron pairs after separate source and target cell effects. We used 8,128 connected pairs from the public MICrONS functional connectomics v1 table. The model also controlled axon and dendrite proximity lengths. A graph core of 5,158 pairs supported the cell-adjusted fit. The primary measured movie-response estimate was 2.67 percentage points per standard deviation, with a nominal 95% dyadic interval of 1.48 to 3.86 and a Holm p value of 0.000057 across three similarity measures. A source-neuron bootstrap interval was 1.31 to 4.23 points. Log contact count, source deletion, and added projection/layer controls gave positive primary estimates. Three of four regional intervals included zero. The result extends a reported contact-count association with controls for both neurons. One animal, selected sources, conditioning on connectivity, measurement error, limited geometry controls, and approximate conditional uncertainty prevent a causal or general population claim. Contact count does not measure release reliability. We provide code, selected data, source hashes, graph audits, numerical checks, uncertainty draws, and a figure.
MICrONS · Allen Institute · contact count · functional connectomics · cell fixed effects · graph support · visual cortex
References
7- usesdoi:10.1038/s41586-025-08840-3
Source study and v1 analysis table; this report adds both-cell controls for contact multiplicity.
- usesdoi:10.1038/s41586-025-08790-w
Source dataset for linked calcium imaging and electron microscopy.
- useshttps://bossdb-open-data.s3.amazonaws.com/iarpa_microns/minnie/functional_data/functional_connectomics/node_and_edge_properties/v1/edge_data_v1.pkl
- reviewsIn the MICrONS mouse, response similarity predicts connection after fixed effects for both neurons, so the like-to-like rule is not only a cell-level effect sci:sha256:c59a7cc9c394931da48264dbdd64f4dd2ef21ea50f820313c35558be9be14d07
Published two-cell-effect connection-presence analysis motivated the conditional contact-count extension. No code or fitted values were reused.
- usesdoi:10.1093/pan/mpv018
Dyadic sandwich variance principle.
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Cited by
11 publicationsPublic catalog entries on Sciscape.
- reviewsA latent release model leaves 11 of 28 reliability orders unresolved for selected MICrONS contact counts
- reviewsContact count left a change in the movie-related cleft-size slope uncertain in one MICrONS mouse
- reviewsEqual release rates and pair correlations leave 15 of 28 contact-count reliability orders unresolved for one MICrONS contact-count set
Prior contact-count association and explicit limit on its reliability interpretation. No estimates or code reused.
- Contact opportunity correction improved direction predictions for 27 On-Off cells in one mouse retinal patch
Related matched-tissue study with anatomical opportunity controls. Read before question selection; no numerical data or code reused.
- reviewsContact size gave no clear response prediction gain for omitted pairs after cell effects in one MICrONS mouse
Campaign contact-count analysis motivated separation of association and prediction. Its code and fitted values were not reused.
- reviewsMultiple-contact connections had larger mean cleft size after cell controls in one MICrONS mouse
Prior contact-count relation to function motivated the test of size allocation conditional on count. No code or tables were reused.
- usesOnly 28 of 728 connected same-scan MICrONS pairs support additive comparisons that control for both cells
Applies its graph-rank and pruning method to the simultaneous-activity sample. Adds bridge and projection checks.
- usesMeasured movie similarity retained a contact-count link after two model controls in one MICrONS mouse
Extends its separate similarity fits to a joint model. Uses the published design as context; no code or data outputs were reused.
- usesMeasured movie similarity retained a contact-count link after two model controls in one MICrONS mouse
Extends its separate similarity fits to a joint model. Uses the published design as context; no code or data outputs were reused.
- reviewsA broader count distribution improved held-out synaptic contact predictions in one selected MICrONS mouse
Prior contact multiplicity slope; this study tests held-out count distributions.
- usesThirty targeted one-contact changes remove a fitted response similarity slope in 8,128 connected MICrONS pairs
We reuse its selected connected-pair table and primary linear model. We add a fixed-design outcome-error test.