A fixed test found no clear sister cell advantage in odor response change agreement across separate intervals in two mice

We tested whether a traced common glomerulus marks odor response changes that agree across separate repeat intervals. We used four rounds of calcium responses to 47 odors in each of two anesthetized mice. The primary cohort had 246 sister pairs in 38 glomerular groups. We compared round 2 minus round 1 with round 4 minus round 3. We removed plane means and compared sisters with other cells in the same plane. The sister advantages were -0.0355 and +0.0487 correlation units. The equal-mouse mean was +0.0066. Its anatomical label and round order upper-tail fractions were 0.3649 and 0.4000 in 9,999 draws. A descriptive group resample range was -0.0164 to +0.0302. It does not measure uncertainty across mice. A later comparison within the individual intervals gave positive advantages of +0.2064 and +0.1342. Thus, the fixed test gave no clear mean sister advantage across separate intervals, despite positive within-interval associations. Self-agreement of changes was low. The result does not establish that persistent neural changes are absent. Synthetic and added-signal checks confirmed that the calculation can detect a common linear change under the stated models. Every compact response mean and anatomical label matched the primary source arrays exactly. A clean run from rebuilt inputs reproduced the numerical analysis and validation outputs. The package preserves source code, compact data, source hashes, all reference draws, figures and repeat instructions.

olfactory bulb · anatomical sister cells · common input · calcium imaging · response change · repeat intervals · X-ray tracing