With at least ten measured trains, 30 of 175 mouse V1 output pairs reversed response order at 50 Hz; six reversals survived a 10% plus 20 µV error bound

We tested whether repeated spikes preserve the response strength order of two outputs from one neuron. In Allen mouse V1 slice data, 30 of 175 output pairs reversed measured order between the first response and pulses 6–8 at 50 Hz. Each connection had at least ten accepted first and late observations. Six reversals remain when each amplitude error is bounded by 10% of its measured magnitude plus 20 microvolts. This bound is an assumption, not a confidence limit. We give an exact uncertainty rule and all pair scores. In separate 30 Hz mouse V1 data, ten of 100 output pairs reversed their first-to-second local voltage order. Three kept the same opposite orders in both alternating trial halves. Measuring the second peak from the original baseline gave ten reversals in the same 100-pair panel, but only four overlapped with the local-baseline reversals. In 144 annotated connections from 24 mouse labels, separate connection means gave no clear repeat prediction gain over a rank-one output model. The gain was −2.90%, with a conditional 95% mouse range of −14.19% to +5.38%. These selected physiological graphs are not complete anatomical connectomes. The 50 Hz source lacks raw events, and the two datasets use different response measures. The results show observed changes in output order and the limits of treating small changes as reliable routing effects. The package preserves code, repeated-trial values, selection IDs, figures, provenance and repeat steps.

short-term synaptic plasticity · output strength · mouse visual cortex · connectome · measurement error · repeated trials