Mean SEEG connectivity did not measurably change after negative motor response stimulation in 21 patients

This study tested whether high-frequency stimulation trains that evoked negative motor responses (NMRs) changed mean 1–40 Hz bipolar SEEG channel-pair correlation during 0.5–5 s after stimulation, and whether the change differed by hemisphere. The analysis used all 35 trains in 21 patients from OpenNeuro ds006519. A preregistered plan was written and hashed before signal inspection. The patient-level post-minus-pre mean absolute Pearson correlation change was +0.0058 (95% bootstrap CI −0.0030 to +0.0146; sign-flip p = 0.224; Holm p = 0.75); baseline mean correlation was 0.204. No test had a Holm-adjusted p below 0.05. The post-minus-pre change relative to prestimulation drift was −0.0167 (13 trains, 9 patients; p = 0.383); ipsilateral-minus-contralateral pair change was +0.0013 (11 trains, 6 bilateral patients; p = 0.812). Ipsilateral-minus-contralateral log10 power change was +0.0530 (p = 0.188). The findings do not support a large, network-wide lasting connectivity change at this time scale. The study is limited by the small patient count, sparse bilateral coverage, lack of non-NMR control trains, curator-selected channel subset, and use of one connectivity measure. The dataset is CC0; the archived plan, code, sidecars, hashes, and results are included. The 35 signal files (595 MB) are not included; their SHA-256 hashes and the public dataset source are recorded.

stereo-EEG · SEEG · negative motor response · intracranial stimulation · functional connectivity · Pearson correlation · OpenNeuro · epilepsy