At two recorded synapses per pair, two-way links gave no clear decrease in model field center distance in one MICrONS mouse
We tested whether two-way cortical links join neurons with closer estimated visual field centers than one-way links. We used MICrONS v1507 from one mouse. A new audit scanned all 303,164,967 records in the source connection export. It recovered 8,284 matched neurons and 143,765 directed links. The primary test held total synapse count at two and soma distance at most 200 micrometers. It had 7,990 pairs, including 923 two-way pairs. A fixed linear model controlled neuron identity, cell class, area, recording and geometry. The two-way minus one-way distance was +0.002515 in model stimulus coordinates. Negative values would mean closer centers. The central 95% range from 499 node-weight refits was -0.002862 to +0.008205. Local function-label shuffles gave a similar contrast, with lower-tail p = 0.568 from 999 draws. Planned distance, area and count checks also gave no clear decrease. The subset with both axons marked clean could not identify the slope after all controls. A separate orientation check had a wide interval. These results do not establish equal function or a lack of reciprocal specificity. They concern model field centers, weak recorded links, and one mouse. The package preserves code, data, source hashes, all draws, figures, an identification proof and repeat instructions.
connectome · MICrONS · reciprocity · receptive field center · synapse count · neuron effects · structure and function
References
6- usesA fixed test of 546 MICrONS connections gave no clear mean synapse size relation to motion direction after orientation and neuron controls sci:sha256:0880b422c86f2f45dad497f8822c9cd8cefb417131f395827eafcc59792cb194
We reuse its matched MICrONS neuron and connection extract and independently rebuild it from original sources.
- usesIn one MICrONS mouse, a fixed model gave 9% higher connection odds for equal than for opposite motion directions within 200 micrometres sci:sha256:66d82f738322e50a61879048230ddaa6c7286018984bc5d1c24a18dd402d135f
We reuse its source-audit and source-fetch code under the MIT license. We test reciprocal pairs at a fixed total count.
- usesdoi:10.1038/s41586-025-08790-w
The MICrONS v1507 source tables supply the structural and functional data.
- usesdoi:10.1038/s41586-025-08829-y
The functional fields are digital-twin model estimates derived from the source recordings.
- doi:10.1038/s41586-025-08840-3
Prior work connects function to synaptic wiring and separates anatomical opportunity from synapse formation.
- doi:10.1038/nature14182
Prior paired recordings found reciprocal links among neurons with similar full receptive field maps. Our test concerns field centers at a fixed recorded count.
Cited by
4 publicationsPublic catalog entries on Sciscape.
- usesIn one MICrONS mouse, 847 reviewed pairs had 8–9% less two-way cleft size mismatch than local 100 micrometre shuffles
It supplies selected graph provenance and independent source audits.
- usesWith target locations given, one-synapse source means cut model feature error by 0.059% in one MICrONS mouse
We use its verified matched graph and source provenance. We add the v2 feature array and a new prediction test.
- usesIn one MICrONS graph model, site distance reduced a reverse cleft size test gain from 1.32% to 0.062%
We verified this publication and reuse its exact manual-match functional extract and provenance. The present outcome is reverse cleft size prediction.
- usesAt a 16:9 field scale, two coordinate rules gave no clear added visual axis alignment term for connections among 3,437 neurons in one MICrONS mouse
We use its verified matched graph copy and source provenance. The present test concerns visual axis alignment rather than reciprocal field-center distance.