Spatial blocks reduced the repeated target-pair excess in two adult worm brain maps

We tested whether repeated target grouping at chemical synapses remains above fixed-count control means after spatial blocks. We used 6,421 selected sites in two public adult C. elegans brain maps. Each control kept the target count of every site and the count of each target within each source and spatial cube. At the planned 5 micrometre cube size and zero offset, observed scores were 1.367 and 1.431 times the control means. Without spatial blocks, the ratios were 2.171 and 1.980. All tested local ratios exceeded one. Cube size and offset changed the effect size. These are conditional finite-chain reference results. Cubes do not preserve exact neurite contact opportunity. Two partial maps, annotation uncertainty and no linked activity data limit inference. The result does not establish partner choice, shared release or reliable neural coding.

C. elegans · polyadic synapses · local geometry · fixed margins · target grouping