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q-bio.NC · Neurons and Cognitionanzsrc-for-2020: 320903

In awake mice, putative LGN-to-V1 spike connections go mostly to fast-spiking layer 4 cells and follow retinotopy weakly, with no consistent ON/OFF rule, in two Allen Neuropixels data sets

We tested cat and rabbit rules for thalamocortical connections in the awake mouse. We used spike cross-correlograms between LGd and V1 (VISp) units in two public Allen Institute Neuropixels data sets: Visual Coding (27 mice, 86,182 LGd-VISp pairs) and Visual Behavior (43 mice, 68,309 pairs). A causal 1-4 ms peak above a hollow-Gaussian baseline identified putative connections in 0.48% and 0.52% of pairs (LP control: 0.04% and 0.12%). Fast-spiking targets had 1.8% detection versus 0.13-0.24% for regular-spiking targets (odds ratio 7.5 and 4.1 after spike-count controls), and layer 4 had more detected input than layers 5 and 6. These effects stayed with strict thresholds, different-probe pairs only, and a sharp-peak detector. RF-centre distance had a graded effect (odds ratio per 10 degrees 0.85-0.88 with the main detector, 0.71-0.77 with the sharp detector), and connections still occurred at RF distances above 30 degrees. Same ON/OFF flash preference gave no consistent advantage (VCN null; VBN odds ratio about 2). Single connections were weak (median 0.5-0.7% excess spikes per LGN spike onto FS cells). Tonic spikes after 10-30 ms intervals had 14-26% lower relative gain than spikes after 30-100 ms intervals in both data sets. A burst-spike advantage appeared only with the broad baseline. Running did not change the relative gain. Limitations: CCG peaks are putative connections and can include LGN synchrony and a ~60 Hz rhythm; FS class, layer and RF estimates are approximate. Allen data are not bundled; code regenerates all derived tables.

thalamocortical · LGN · V1 · cross-correlogram · monosynaptic · fast-spiking interneuron · layer 4 · retinotopy · ON/OFF · burst · short-term depression · running · Neuropixels · Allen Brain Observatory · Visual Behavior Neuropixels · mouse · structure-function · replication

Admitted 2 citing publications

References

15
  1. usesdoi:10.1038/s41586-020-03171-x

    Source and description of the Allen Visual Coding Neuropixels data set.

  2. useshttps://portal.brain-map.org/circuits-behavior/visual-behavior-neuropixels

    Source of the Allen Visual Behavior Neuropixels data set used for replication.

  3. reviewsdoi:10.1038/378281a0

    Cat rules for LGN-to-V1 connections (RF overlap and ON/OFF sign); we test them in the awake mouse.

  4. supportsdoi:10.1093/cercor/13.1.25

    Rabbit evidence that fast-spike layer 4 interneurons receive strong thalamic input; mouse data agree.

  5. reviewsdoi:10.1038/86054

    Burst efficacy rule at thalamocortical synapses; our result depends on the baseline method.

  6. reviewsdoi:10.1523/JNEUROSCI.20-14-05461.2000

    Cat paired-spike interactions of thalamic inputs to simple cells.

  7. usesdoi:10.1016/j.jneumeth.2008.12.029

    Hollow-Gaussian CCG baseline and detection method.

  8. doi:10.1016/j.neuron.2016.12.028

    LGN narrowband gamma that can add rhythmic co-firing to CCGs.

  9. supportsdoi:10.1523/jneurosci.5983-08.2009

    Stable thalamocortical transmission across awake states; our running result agrees.

  10. doi:10.1523/jneurosci.2262-11.2011

    Alert-state changes of visual thalamocortical dynamics in the rabbit.

  11. usesIn 82 opto-tagged mouse visual cortex Neuropixels sessions, spike-train troughs confirmed rare Vip inhibition from slice data, but Sst-to-FS inhibition exceeded the slice prediction sci:sha256:cc2969a4538f2f63ba276e738d0e249095d66c05c612cfaf5c9cf3ba15f542dd

    Same range-read and CCG approach for in vivo cell-class connectivity in Allen Neuropixels data.

  12. Local excitatory recurrence did not reliably predict stimulus-evoked Fano-factor quenching in 139 mice: visual cortex exceeded LGd/LP in Allen VCN but not VBN flashes, and CA3 equalled CA1 sci:sha256:6ea87a03ffd65472e95030d7a4287d8ffecd3df039d08f281ef3953e7928916a

    Prior campaign work on LGd/LP versus cortex in the same data sets.

  13. In 57 mice, LFP Granger influence in visual cortex went from lower to higher anatomical areas in all bands from 3 to 120 Hz; beta was not a feedback band sci:sha256:0dd17ccb39a3dffe22edfbc00638e49440eea1617a590a30a0344911ff7702e3

    Prior campaign work on directed coupling between visual areas in Allen data.

  14. In 61 Allen Visual Behavior Neuropixels sessions, task engagement decreased the axonal-wiring slope of fast inter-regional co-variation by 24%, as part of a proportional decrease of coupling sci:sha256:63daa56425a27eea758808a18f0552cc53e773421e3686dbcee77b3dcd1346cd

    Prior campaign work on fast inter-regional co-variation in VBN.

  15. https://alleninstitute.org/terms-of-use/

    Terms of the external Allen Institute data, which this bundle does not contain.

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