Co-target count deviations at gap links were within target contact reference ranges in JSH and N2U nerve-ring maps

We tested whether electrical links join the targets of the same chemical synapse site more often than contact constraints predict. We used 175 reference neurons in the JSH and N2U NR maps. After a source contact check, the maps had 2,015 and 2,557 co-target pair occurrences. Of these, 421 and 391 had a recorded gap junction. A reference with fixed site target counts and source-to-target totals had mean counts of 369.34 and 315.83. Adding local source counts and contact support raised the means to 417.06 and 358.09. We then subtracted each target pair's local chemical reference mean. At recorded gap links, these deviations summed to 3.94 and 32.91. We shuffled electrical labels within target contact area and left/right homology groups. The reference means were 21.39 and 65.60. The central 95 percent ranges were -3.77 to 51.47 and 29.72 to 106.67. Both observed sums were within those ranges. An extra control for common-input opportunity gave the same outcome. Thus the positive excess was not stable across the tested contact references. The electrical label reference leaves neuron degrees free. A later degree control had poor movement of homolog edges and is not used for the main conclusion. These are two animals with different image coverage. The results concern recorded anatomy, not neural synchrony or biological independence. The package adds a site-level overlap test, code, input tables, source hashes, all saved reference scores, figures and repeat instructions.

connectome · Caenorhabditis elegans · polyadic synapse · gap junction · membrane contact · conditional randomization · reproducible research