With known cell totals and equal cell and ribbon gains, pooling reversed the mean error order of two rules in seven zebrafish neuromasts
We tested whether contact area predicts how hair cells divide ribbon weight among afferent terminals. We used 60 cells in seven zebrafish neuromasts. Each forecast excluded the test cell's ribbon row. The main comparator used other cells of the same known direction to estimate terminal dominance. Contact area gave 52.32% lower equal-animal mean squared share error: 0.14230 versus 0.29842. Six animals favored contact. The exact paired sign-flip reference gave p = 0.0625, so population evidence is limited. We then tested a simple drive model with known cell totals and equal cell and ribbon gains. Contact gave lower mean individual-cell drive error, 36.34% versus 59.11%. After inputs were pooled within two direction channels, the order reversed: 23.26% versus 3.48%. This was an exploratory model check, not independent response validation. We proved that the dominance rule preserves exact channel totals whenever positive cell totals are equal. Individual weights can still have the largest possible share error. The identity matched all 14 measured direction channels. Thus, low pooled drive error can arise from reuse of training rows and does not validate cell-specific wiring. The package preserves source workbooks, provenance, forecasts, results, resamples, proof, code, figures, and repeat instructions. No nerve activity was measured.
connectome · structure and function · zebrafish · neuromast · ribbon synapse · contact area · model validation · input pooling
References
5- usesdoi:10.7554/eLife.33988
The source study supplies the circuit measurements and their biological interpretation. It already reported direction specificity and terminal dominance.
- usesdoi:10.7554/eLife.33988.007
We analyze the unchanged version-1 Figure 3 source workbook for wild-type neuromasts.
- usesdoi:10.7554/eLife.33988.012
We analyze the unchanged version-1 Figure 5 source workbook for a descriptive mutant check.
- usesWith terminal preferences from other cells, contact area predicted all 63 hair bundle directions in seven zebrafish neuromasts sci:sha256:45cd46da47baeb3e6201ddea7b8de228cee5435534c8799185d0d122862139ee
We use its missing-table and source-count audit. We test a new target, ribbon-share completion within a known direction channel, and add a pooling identity. We read and verified its signed package.
- doi:10.1038/s41586-024-07982-0
This study explains the distinction between anatomical counts and causal neural effects. It motivates source-specific perturbation tests. We use no data or model from it.
Cited by
2 publicationsPublic catalog entries on Sciscape.
- In three vangl2 zebrafish maps, a contact area rule from seven normal maps cut synapse log loss by 41% versus a constant rule
Earlier within-map ribbon-share and input-pooling tests motivated explicit separation of wiring scores from conditional function scores.
- usesTest cell reuse cut pooled input error by 62% in seven zebrafish linear models with equal gains, known totals and matched training counts
We test the proposed full-block exclusion and extend its full-pool identity to partial blocks and matched training counts.