With terminal preferences from other cells, contact area predicted all 63 hair bundle directions in seven zebrafish neuromasts
We tested whether ribbon counts improve direction prediction beyond membrane contact area in zebrafish sensory circuits. The main sample had 63 hair cells from seven wild-type neuromasts. Each test direction was excluded from terminal preference estimates. A fixed rule used ribbon counts from other cells to assign terminal preferences. Contact area then classified all 63 directions. Binary contacts gave 76.84 percent equal-animal accuracy. Ribbon and contact mean squared probability errors were 0.020705 and 0.035758. The contact-minus-ribbon difference was 0.015053. Its descriptive 95 percent animal resample range was -0.006235 to +0.041571. The paired sign-flip reference gave p = 0.296875. Thus, the primary comparison gave no clear extra ribbon benefit across animals. The least certain correct contact forecast assigned probability 0.511211 to its true class. Using contact area for both preference assignment and prediction gave 94.39 percent accuracy. A separate eighth wild-type circuit lacked contact data. Four mutant circuits gave variable continuous-direction results. The source already reported direction-specific contacts and synapses. We add a test with each target label excluded, weighted and binary controls, and a reproducible benchmark. The result concerns missing labels within measured circuits. Bundle directions indicate mechanical sensitivity, but no nerve activity was measured here. The package preserves source workbooks, provenance, extracted tables, all forecasts and resamples, code, figures, validation, and repeat instructions.
connectome · zebrafish · neuromast · mechanosensation · membrane contact area · direction prediction
References
3- supportsdoi:10.7554/eLife.33988
The source study reported direction-specific synapses and membrane contacts. We test its contact specificity with each target direction excluded from terminal preference estimates.
- usesdoi:10.7554/eLife.33988.007
We use the version 1 Figure 3 source workbook for eight wild-type neuromasts. Exact URLs and SHA-256 hashes are preserved.
- usesdoi:10.7554/eLife.33988.012
We use the version 1 Figure 5 source workbook for four trilobite mutant neuromasts in a secondary direction analysis.
Cited by
4 publicationsPublic catalog entries on Sciscape.
- In three vangl2 zebrafish maps, a contact area rule from seven normal maps cut synapse log loss by 41% versus a constant rule
Earlier within-map direction-label prediction provides task context; our outcome is synapse presence in complete test maps.
- usesWith two direction groups assumed and one label per graph, contact area classified 54 of 56 other hair cells in seven zebrafish neuromasts
Its earlier direction benchmark motivates the new test with only one known label per graph. New inputs are read from the primary workbook.
- usesTest cell reuse cut pooled input error by 62% in seven zebrafish linear models with equal gains, known totals and matched training counts
Its source audit informed the sample and missing-table treatment. We wrote a new reader and checked source values independently.
- usesWith known cell totals and equal cell and ribbon gains, pooling reversed the mean error order of two rules in seven zebrafish neuromasts
We use its missing-table and source-count audit. We test a new target, ribbon-share completion within a known direction channel, and add a pooling identity. We read and verified its signed package.