In 19 mice, sums of two spine calcium signals gave no clear local advantage in a linear orientation test
We tested whether nearby synaptic inputs support a simple local readout in mouse visual cortex. We used measured calcium amplitudes from the public Jenks dataset. The main sample had 6,073 near pairs and 7,773 far pairs on the same branch. Near distances were 2 to less than 5 micrometres. Far distances were 10 to less than 20 micrometres. It included 22 neurons in 19 mice. Five-fold tests compared a sum of two normalized signals with a readout that kept both signals separate. Both readouts predicted doubled-angle orientation coordinates by ridge regression. The near-minus-far pooling cost was 0.00050, with a 95% mouse bootstrap interval of -0.00117 to 0.00221. Trial pairing shuffles and planned alternate tests gave similar limits. Broad-sample decoding scores were close to the zero predictor. Controls added after the main result found readable signal in source-selected responsive spines and detected an imposed local tuning pattern. Thus, the specified linear readout gave no clear local advantage in the broad spine sample. Low scores and only three eligible mice with responsive pairs limit biological inference. The study does not test nonlinear dendritic integration or measured synaptic currents. The package includes checked input arrays, code, all pair scores, source hashes, figures, and repeat instructions.
dendritic spines · visual cortex · orientation · linear readout · calcium imaging · local input groups · trial covariance · reproducible benchmark
References
6- usesIn 10 mice, clipping trial amplitudes at zero cut the <5 versus 10–20 µm tuning correlation contrast of responsive visual cortex spines by 0.085 in tests with odd and even repeats sci:sha256:fe21349f81db26fc0fed7410847f79826d047b45849490025a94aafa867af7e2
We use its checked compact signed response arrays and source extraction code. We verified its signed package and matched all 22 input file hashes.
- usesdoi:10.5281/zenodo.19372968
This version 1.0 dataset supplies the measured spine responses and structural distances.
- doi:10.1016/j.isci.2026.115861
This source study measured spine responses and reported local tuning organization.
- doi:10.1038/nn.4323
This study linked local spine tuning groups to nonlinear dendritic responses.
- doi:10.7554/eLife.46966
This study separated local tuning similarity from shared trial fluctuations.
- doi:10.3389/fnsyn.2022.888214
This study used population decoding to examine diverse synaptic inputs.
Cited by
3 publicationsPublic catalog entries on Sciscape.
- Equal averages of nearby spines gave no clear extra agreement between orientation signals from the two eyes in 11 mice
This prior study tests linear orientation readout from spine pairs under binocular stimulation. Our test concerns agreement between separate eye signals after an equal average.
- A fixed rank test found no clear extra calcium coincidence in spine triplets within 5 µm in 18 mice
This prior work tested a linear sum of two spine signals. The present test measures joint high ranks in three signals.
- In 19 mice, sums of two spine calcium signals 2–5 µm apart on the same branch lost 36% of tuning energy with asinh scaling and independent repeat errors
Its fitted pooling readout supplies prior results. This study adds a model-free tuning-energy decomposition in a fixed signal scale.