At a 500 nM cutoff, the two-way edge fraction between expression groups was within a matched receptor profile range in the 2024 C. elegans short-range peptide map
We tested whether two-way peptide links exceed a reference that keeps complete receptor profiles and expression breadth. We used the February 2024 C. elegans map, 302 neuron labels, 127 distinct source expression groups, and 92 molecular gene pairs at 500 nM. The main short-range graph excluded pairs within the same expression group. A control exchanged whole receptor profiles only between groups with equal receptor gene counts and equal neuron copy counts. This kept receptor gene frequency and co-expression at both group and neuron levels. The graph had 30,824 directed edges and 9,736 unordered two-way pairs. The fraction of edges with a reverse edge was 63.17%. In 20,000 independent assignments, its mean was 62.52% and its central 95 percent range was 60.87–64.14%. The two-sided reference tail value was 0.437. Each of seven planned checks also placed the fraction inside its reference range. At 100 nM, the edge count exceeded its reference range, while the two-way fraction did not. Exact count means, exhaustive small cases, all saved scores, full adjacency checks, and a clean numerical repeat passed. This result concerns a conditional expression model. It does not establish equivalence, physiological feedback, or variation between worms. The package preserves source files and hashes, code, all 160,000 assignments, results, figures, and repeat instructions.
Caenorhabditis elegans · neuropeptide · connectome · reciprocity · gene expression · receptor profiles · conditional randomization · reproducibility
References
7- usesdoi:10.1016/j.neuron.2023.09.043
The source study supplies the peptide map, molecular layers, and anatomical range rules. We test its February 2024 Git update under a different reference.
- usesdoi:10.1016/j.cell.2021.06.023
CeNGEN supplies the expression resource used by the peptide map. We use its released expression profiles to identify repeated source profiles.
- usesdoi:10.1016/j.celrep.2023.113058
The source interaction screen supports the molecular gene pairs used by the peptide map.
- usesdoi:10.6084/m9.figshare.c.6895870.v1
Archived source study collection. The exact later map bytes used here are pinned by Git commit and SHA-256, not by this collection DOI alone.
- usesPredicted self-signaling pair counts fell within matched receptor profile ranges in 127 C. elegans expression groups sci:sha256:70c513a1b25c8f973e923136e6aae7e5ea6deff017b8baaf05011f76e608ea52
We build on its whole receptor profile exchange. We add neuron copy matching and test reciprocal intergroup edges rather than diagonal self-pair counts.
- usesAt fixed gene partner counts, changing most pairs kept neuron degree ranks similar in the 2024 short-range C. elegans peptide map sci:sha256:ad48c7fa79305861e670b50997a255aa8e7fe74149ae6dcebbeb3acfae360218
We reuse its MIT source preparation script. It tests molecular partner changes and hub ranks, while this work tests receptor assignment and reciprocal edges.
- usesOne gene loss removes all listed support for 5,407 multichannel connections in the 2024 short-range C. elegans peptide model sci:sha256:ca108f7e42c2e47e88eeb5d6ad7b4aaa0d7a256d19e214b2bc1d3ef954ae4367
We use the unchanged versioned shared source cache created for this study. Its gene deletion results are not reused.
Cited by
1 publicationPublic catalog entries on Sciscape.
- usesAt 500 nM, high-degree half connection concentration was below a matched receptor profile range in the 2024 C. elegans short-range peptide map
We reuse prepared arrays, source grouping, the copy-count matched whole receptor profile reference, and the attributed preparation code. We test core concentration instead of two-way edge fraction.