Predicted self-signaling pair counts fell within matched receptor profile ranges in 127 C. elegans expression groups
We tested whether predicted peptide self-signaling has a global count excess in a published C. elegans map. We used 92 known gene pairs and the February 2024 source update. We grouped 302 neuron labels into 127 distinct source expression profiles. Each random assignment exchanged whole receptor profiles among groups with the same receptor gene count. It kept peptide profiles, receptor frequencies, and receptor co-expression fixed. The observed total was 246 cognate self-pair records. The exact reference mean was 252.03. The central 95 percent range from 20,000 assignments was 231 to 273. The two-sided p value was 0.614; the Holm value for two statistics was 1.00. Eighty units had one or more records, compared with a reference mean of 82.06. Cell type, receptor removal, and anatomical class controls gave similar count comparisons. Without receptor count matching, the expected total was 230.33. The observed count lay within both ranges. This fixed-map test did not detect a global excess under the stated profile exchange assumption. It does not test neural activity, prove equivalence, or measure variation between worms. The package preserves source data and hashes, code, all assignments, results, figures, and reproduction commands. Exact rational checks, exhaustive small cases, and a fresh source rebuild passed. This record corrects a related SCI web link in the first version. The data and numerical results match that version.
Caenorhabditis elegans · neuropeptide · autocrine signaling · gene co-expression · conditional randomization · connectome · reproducible methods
References
6- usesdoi:10.1016/j.neuron.2023.09.043
We use its public neuropeptide map and test its predicted self-pair counts with a matched receptor profile reference.
- usesdoi:10.6084/m9.figshare.c.6895870.v1
This is the source data collection. The package records the exact February 2024 Git source version and file hashes.
- usesdoi:10.1016/j.cell.2021.06.023
CeNGEN supplies the source expression profiles, as released by the neuropeptide map authors.
- usesdoi:10.1016/j.celrep.2023.113058
The known peptide and receptor gene pairs come from this molecular interaction screen through the source map.
- One gene loss removes all listed support for 5,407 multichannel connections in the 2024 short-range C. elegans peptide model sci:sha256:ca108f7e42c2e47e88eeb5d6ad7b4aaa0d7a256d19e214b2bc1d3ef954ae4367
This related study tests gene loss in the same source release. It excludes self connections. Our test concerns self-pair counts.
- supersedesPredicted self-signaling pair counts fell within matched receptor profile ranges in 127 C. elegans expression groups sci:sha256:fd0910163a8ff2cde5a8dd7b21563f2140ed3f57d518d00e38cde3690faad633
This record corrects the web address for the related SCI study in the main text. The data and numerical results match the first version.
Cited by
8 publicationsPublic catalog entries on Sciscape.
- supportsRemoving 171 self entries raised rank from 38 to 202 in the 2024 long-range C. elegans peptide count map at 500 nM
The prior study reports 509 self-pair records in 171 neuron labels from the same source update. Our study tests matrix rank and exact contrast modes.
- usesAt a 500 nM cutoff, the two-way edge fraction between expression groups was within a matched receptor profile range in the 2024 C. elegans short-range peptide map
We build on its whole receptor profile exchange. We add neuron copy matching and test reciprocal intergroup edges rather than diagonal self-pair counts.
- usesA peptide match count gave no stable prediction gain in fixed tests of 1,278 C. elegans response directions
We reuse its binary molecular expression, gene-pair and identical-profile group extracts. Fresh original layers and continuous expression verify each extract.
- usesPeptide pair counts raised ridge test error by 0.20% for WT and unc-31 response differences in 1,342 C. elegans atlas pairs
We reuse binary gene expression, known gene pairs, and continuous expression-profile groups.
- Single-gene losses removed all two-step routes for 6,714 pairs with several relays in a fixed 2024 C. elegans short-range peptide model
This prior analysis documents shared expression profiles in the peptide map. We derive channel role profiles independently.
- usesKnown peptide–receptor matches did not clearly exceed controls with fixed gene degrees for wild-type minus unc-31 detection in 1,342 C. elegans atlas directions
We reuse verified numerical binary expression, molecular pairs and the 127 groups with distinct source expression profiles.
- usesAt fixed gene partner counts, changing most pairs kept neuron degree ranks similar in the 2024 short-range C. elegans peptide map
Its source-data checks guided independent reconstruction and the 127-profile control. No analysis code was copied.
- usesPeptide channel count gave no clear gain in a fixed ridge test of WT minus unc-31 detection for 3,261 C. elegans atlas pairs
We use its checked source release, provenance and molecular layer definitions to build a new join to evoked neural responses.