With experiment and cell labels fixed, shared targets gave no clear extra similarity in initial 50 Hz changes in 70 mouse and 39 human cortical connections

We tested whether excitatory connections to the same cortical neuron have more similar initial 50 Hz response changes. We used all available projects in Allen Synaptic Physiology release r2.1. The source contains small recording graphs from brain slices. It is not a complete anatomical connectome. Of 438 eligible mouse and 121 human connections, 70 and 39 entered informative local comparisons. Each local group fixed the experiment, both Cre labels, both layers, and the independent target class. We compared the mean squared difference for shared-target pairs with that for all connection pairs in the group. We averaged groups within donors, then across donors. The ratios were 1.200 in mouse and 0.970 in human. Their 95 percent donor bootstrap intervals were 0.920–1.475 and 0.536–1.437. Random assignment of outcomes within each fixed graph gave two-sided p values of 0.263 and 0.892. No clear extra similarity remained under this comparison rule. With the same local groups and weights, a reference from other experiments gave ratios of 0.845 and 0.375, against local ratios of 1.219 and 0.970. This reference check is descriptive. All human test groups had excitatory targets. Simulations on the observed graphs showed limited sensitivity to moderate target effects. The result does not establish equal input dynamics or a causal target effect. The contribution includes a local permutation method, graph and source audits, aggregate results, figures, and code for a full rebuild.

connectome · short-term plasticity · convergent connections · cortical circuits · multipatch · structure and function · permutation test · Allen Synaptic Physiology