Initial 2–5 µm neighbor links gave no clear extra agreement in five-day binocular tuning changes of retained spines in nine mice
We tested whether retained neighboring spines change visual tuning together over five days. The data came from released mouse visual-cortex calcium recordings. The primary test used 42 initial neighbor links from 2 to less than 5 micrometres apart, 255 retained spine intervals, 14 cells and nine mice. Separate odd and even stimulus repeats gave a local change score of 0.0391. Its 95% mouse bootstrap interval was -0.0244 to 0.1235. A within-field label test gave one-sided p = 0.0998. Removing additive endpoint terms gave 0.0174, with an interval from -0.0299 to 0.0745. An added analysis of all distinct repeats found individual change energy of 0.0654 [0.0287, 0.1088], while local excess remained uncertain at 0.0270 [-0.0359, 0.1140]. Thus, the test did not establish extra local agreement despite measurable individual changes under independent-repeat error assumptions. It does not exclude local plasticity. Small animal numbers, source-selected neighbors, optical signals and shared preprocessing limit inference. The package preserves compact data, source checks, code, all results, figures and repeat instructions.
dendritic spines · visual cortex · development · structure and function · calcium imaging · tuning changes · repeated measurements · local plasticity
References
6- usesdoi:10.1038/s41467-025-60825-y
Original developmental spine calcium measurements, structural tracking and source methods.
- usesdoi:10.6084/m9.figshare.28738592.v1
Version 1 of the tracked spine table, file 53453786; exact source hash is preserved.
- usesIn 10 mice, equal mouse weights reduced the early binocular response correlation rise from 0.051 to 0.007 in 81 fixed spine pairs sci:sha256:c97d0624765e841d3140bb78b82cd769c0456156be9391a76acdff689c7019f1
Exact compact trial amplitudes, source masks, source rows and numerical neighbor identity recovery. The input bytes match the verified signed archive.
- In 10 mice, clipping trial amplitudes at zero cut the <5 versus 10–20 µm tuning correlation contrast of responsive visual cortex spines by 0.085 in tests with odd and even repeats sci:sha256:fe21349f81db26fc0fed7410847f79826d047b45849490025a94aafa867af7e2
Prior static spine analysis motivated preserving signed trial amplitudes and the specified clipping check. It used a separate biological dataset.
- doi:10.1523/JNEUROSCI.2775-20.2021
Established signal-correlation bias and separate-repeat analysis context.
- usesdoi:10.1214/14-AOS1255
Standard U-centering formula used to remove additive endpoint terms from the pair score.
Cited by
2 publicationsPublic catalog entries on Sciscape.
- usesWith equal spine weights, retained spines gave more summed binocular calcium tuning change than turnover across 13 mice
Verified compact input supplies an exact check of all 8,393 independently rebuilt endpoints. This related report tests local changes only among retained spines; the present comparison includes turnover.
- usesLarger initial spines had more visual calcium tuning energy but no clear five-day stability gain in 13 mice, assuming independent repeat errors
We build on its application of distinct-repeat products to five-day spine tuning changes. We test initial size groups and relative change, rather than shared local changes.