In 19 mice, spine pairs matched on two distances showed no clear branch difference in trial residual calcium correlation
We tested the link between branch points and shared trial fluctuations in mouse visual-cortex spines. We used the Jenks et al. 2026 dataset and signed trial amplitudes from a cited SCI extract. We removed each visual direction's mean response on common accepted trials. We matched spine pairs within each field on path and straight-line distances, each within 1 micrometre. The test used 2,594 comparisons in 102 fields from 22 neurons and 19 mice. The mean no-branch minus branch-point correlation was 0.008. The 95% mouse bootstrap interval was -0.014 to 0.030. The exact sign-flip p value was 0.493. Checks of match width, trial masks, time drift, and distance quality retained an uncertain contrast. A descriptive distance test gave higher residual correlation at 2-5 than 10-20 micrometres. The branch result does not establish equivalence or rule out a modest effect. It concerns processed calcium signals and the subset with geometric overlap. We release fixed matches, checked compact data, independent code, figures, and provenance.
dendritic spine · branch point · structure and function · calcium imaging · mouse visual cortex · noise correlation · distance matching
References
5- usesIn 10 mice, clipping trial amplitudes at zero cut the <5 versus 10–20 µm tuning correlation contrast of responsive visual cortex spines by 0.085 in tests with odd and even repeats sci:sha256:fe21349f81db26fc0fed7410847f79826d047b45849490025a94aafa867af7e2
We reuse its checked compact signed-amplitude arrays and source provenance. We add a distance-matched branch test across all fields.
- usesdoi:10.5281/zenodo.19372968
Version 1.0 provides the original spine anatomy and visual-response data. We check every compact field against the original MATLAB members.
- usesdoi:10.1016/j.isci.2026.115861
The source study defines the recordings and signal processing.
- usesdoi:10.5281/zenodo.18552068
Source code version V1.0 was consulted for data definitions. No original MATLAB code is bundled.
- doi:10.7554/eLife.46966
This study reports branch-related task signal organization in mouse motor cortex and motivates the structural question. Its task, area, and outcome differ from this test.
Cited by
5 publicationsPublic catalog entries on Sciscape.
- In 11 mice, patterns of shared calcium variation between spines repeated across eye conditions after linear controls
This prior trial-correlation study motivates the branch and common-trial controls. Our new test concerns cross-eye map stability.
- In 19 mice, 367 matched spine comparisons gave a shaft-distance calcium correlation contrast of −0.010
It tested branch-point separation at matched path and center distances. It also reported an exploratory local-distance contrast. We test each distance at similar values of the other measure.
- usesA fixed rank test found no clear extra calcium coincidence in spine triplets within 5 µm in 18 mice
We reuse its source audit code and compare the new triplet question with its pair correlation test.
- usesIn 19 mice, sums of two spine calcium signals 2–5 µm apart on the same branch lost 36% of tuning energy with asinh scaling and independent repeat errors
We verify and reuse its copy of the compact input data and its independent source-audit code.
- Adding a spine calcium signal 2–5 µm away decreased linear test error by 0.34% after field and distant signals in 19 mice
Its branch test and descriptive distance result informed our question. We add a prediction test after field activity and a distant partner are known.