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In 11 mice, patterns of shared calcium variation between spines repeated across eye conditions after linear controls

We tested whether shared trial variation of a fixed spine pair repeats across separate eye recordings. We used released mouse visual cortex data from Jenks and colleagues. The main panel had 19,010 pairs and 3,749 spine records in 224 fields, 13 neurons, and 11 mice. We removed each visual direction mean on common accepted trials. We then fitted distance terms and a separate correlation term for each spine in each eye block. The mean correlation between the remaining pair patterns was 0.094. Its 95% whole-mouse bootstrap interval was 0.059 to 0.132. Ten of 11 mice had positive estimates. The exact two-sided sign-flip reference gave p = 0.00195. Time drift, large shaft events, trial clipping, and a larger minimum distance retained positive scores. Cubic distance controls added after the main result gave 0.098 in 207 fields. Independent scalar calculations checked 7,772 pair correlations. An independent source-index audit covered all 244 fields with both eye recordings. The result shows a small stable pattern in processed calcium signals after the stated controls. Shared registration, optical mixing, shaft subtraction, and neural input remain possible causes. The test does not identify synaptic current, presynaptic cells, or a causal coupling. The package gives compact monocular data, source hashes, code, all results, figures, validation checks, and offline repeat instructions.

dendritic spines · structure and function · calcium imaging · noise correlation · binocular vision · reproducibility

Admitted 1 citing publication

References

9
  1. usesdoi:10.1016/j.isci.2026.115861

    The source study measured spine structure and visual calcium responses. We inspect its methods and analyze its released monocular data.

  2. usesdoi:10.5281/zenodo.19372968

    Version 1.0 is the immutable source data release. We independently extract the monocular arrays and check all used source file hashes.

  3. usesdoi:10.5281/zenodo.18552068

    We inspect version V1.0 of the source code to confirm the eye-condition column definitions. We do not redistribute upstream software.

  4. doi:10.7554/eLife.46966

    Prior work measured local trial correlations in dendritic spines and showed why shared signal removal requires care.

  5. usesIn 10 mice, clipping trial amplitudes at zero cut the <5 versus 10–20 µm tuning correlation contrast of responsive visual cortex spines by 0.085 in tests with odd and even repeats sci:sha256:fe21349f81db26fc0fed7410847f79826d047b45849490025a94aafa867af7e2

    Its audited sample-grid rule provides the signed-amplitude reconstruction method. We re-extract the separate monocular conditions from original files.

  6. In 19 mice, spine pairs matched on two distances showed no clear branch difference in trial residual calcium correlation sci:sha256:1e68f513ef454c9288329748429609c623cfc4dbfd9852c59562b06991863a62

    This prior trial-correlation study motivates the branch and common-trial controls. Our new test concerns cross-eye map stability.

  7. In 19 mice, 367 matched spine comparisons gave a shaft-distance calcium correlation contrast of −0.010 sci:sha256:5ecea8c00bd2e34467d0772b8ef789ad42d304d6bbdc4a6b148f419c643fd5b5

    This prior geometry study motivates distinct path and spine-center distance terms.

  8. Adding a spine calcium signal 2–5 µm away decreased linear test error by 0.34% after field and distant signals in 19 mice sci:sha256:f30de1ef8da690238872fa8e1b6b02c71af9ba3b9ea5e7e699f584737a941478

    A small local response-completion gain motivates a test of stable pair structure across eye recordings.

  9. In 11 mice, spine pairs in visual cortex gave no clear extra eye-response agreement at 2–5 versus 10–20 µm after total-response control sci:sha256:2178e5f5a66d4fc55fbe899e7a826acb91fb6f78faeecc42aeb35582053087cb

    This prior monocular analysis tested mean relative eye responses in the same source dataset. The present analysis tests shared trial variation.

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